FastQCFastQC Report
Sun 14 Apr 2019
SRR6576473_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576473_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463091
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14410.311169942840608No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8500.18354923762284303No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8220.17750290979526703No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA8140.17577538755881675No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA7260.15677264295786356No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6820.147271270657387No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA6800.14683939009827443No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG6150.13280327192711583No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT6000.12956416773377155No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5910.12762070521776497No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5690.12287001906752669No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG5340.11531210928305669No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5100.11012954257370582No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4880.10537885642346752No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4850.10473103558479865No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA4820.10408321474612982No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT4780.10321945362790468No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4750.10257163278923581No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4670.10084411055278553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG32050.041.055891
TACATGG32300.040.5926252
ACATGGG32450.040.2514723
GTATCAA12750.039.4383771
CATGGGG10200.035.007824
CATGGGT8400.033.0007934
ATGGGTA5200.032.5275085
AACGCAG15750.031.62116
CTATTAC753.279012E-431.3227863
ATCAACG15650.031.2227133
ATGGGAT5600.031.0431215
CAACGCA15950.030.9300255
TCAACGC16050.030.4445764
CATGGGA18600.029.5545654
GACCGGT650.006160547528.9133427
GCCGCAA650.006160547528.9133423
CATGGGC5100.027.6377534
TGCCCGC700.00883586626.8539031
GTCTAGC700.00883586626.8539031
ATGGGAC4200.026.8481035
Produced by FastQC (version 0.11.2)