Basic Statistics
Measure | Value |
---|---|
Filename | SRR6576474_2.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 321372 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 50 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG | 739 | 0.2299515825896469 | No Hit |
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA | 479 | 0.14904845475025827 | No Hit |
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 466 | 0.14500329835828882 | No Hit |
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA | 397 | 0.12353285289322032 | No Hit |
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA | 388 | 0.12073236000647225 | No Hit |
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG | 362 | 0.11264204722253339 | No Hit |
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT | 350 | 0.1089080567068693 | No Hit |
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA | 344 | 0.10704106144903726 | No Hit |
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC | 340 | 0.10579639794381589 | No Hit |
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG | 333 | 0.10361823680967851 | No Hit |
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA | 328 | 0.10206240742815179 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CCCGAAT | 25 | 0.0052337847 | 56.396824 | 94 |
TATTTCG | 40 | 5.667804E-4 | 47.00467 | 5 |
TAATGTG | 50 | 3.0027879E-5 | 47.004665 | 4 |
GTATCAA | 950 | 0.0 | 45.6197 | 1 |
CTATTCA | 55 | 5.2763236E-5 | 42.724865 | 9 |
GTACATG | 2215 | 0.0 | 40.62074 | 1 |
TACATGG | 2325 | 0.0 | 38.816757 | 2 |
ACATGGG | 2360 | 0.0 | 37.64357 | 3 |
AACGCAG | 1170 | 0.0 | 37.362686 | 6 |
ATCAACG | 1210 | 0.0 | 36.127556 | 3 |
TCAACGC | 1205 | 0.0 | 35.88738 | 4 |
CAACGCA | 1220 | 0.0 | 35.446144 | 5 |
CATGGGT | 570 | 0.0 | 33.81038 | 4 |
CATGGGG | 1065 | 0.0 | 31.777802 | 4 |
ATGGGTA | 385 | 0.0 | 31.743414 | 5 |
ACGCAGA | 1395 | 0.0 | 31.33157 | 7 |
GGTACCT | 300 | 0.0 | 29.764992 | 8 |
TATGGGA | 95 | 3.7414226E-5 | 29.68716 | 2 |
CGCAGAG | 1460 | 0.0 | 29.61477 | 8 |
TATCAAC | 1535 | 0.0 | 29.397057 | 2 |