FastQCFastQC Report
Sun 14 Apr 2019
SRR6576474_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576474_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences321372
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7390.2299515825896469No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4790.14904845475025827No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4660.14500329835828882No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3970.12353285289322032No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3880.12073236000647225No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3620.11264204722253339No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3500.1089080567068693No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3440.10704106144903726No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3400.10579639794381589No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3330.10361823680967851No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3280.10206240742815179No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAAT250.005233784756.39682494
TATTTCG405.667804E-447.004675
TAATGTG503.0027879E-547.0046654
GTATCAA9500.045.61971
CTATTCA555.2763236E-542.7248659
GTACATG22150.040.620741
TACATGG23250.038.8167572
ACATGGG23600.037.643573
AACGCAG11700.037.3626866
ATCAACG12100.036.1275563
TCAACGC12050.035.887384
CAACGCA12200.035.4461445
CATGGGT5700.033.810384
CATGGGG10650.031.7778024
ATGGGTA3850.031.7434145
ACGCAGA13950.031.331577
GGTACCT3000.029.7649928
TATGGGA953.7414226E-529.687162
CGCAGAG14600.029.614778
TATCAAC15350.029.3970572