FastQCFastQC Report
Sun 14 Apr 2019
SRR6576475_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576475_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences291804
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7310.2505106167153295No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4880.1672355416649532No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4230.14496031582843277No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3560.12199969842771176No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3560.12199969842771176No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3410.11685926169620704No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3170.10863456292579951No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3080.1055503008868967No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3060.10486490932269604No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3010.10315143041219449No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2980.10212334306589355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8650.038.0377351
CGAACTC500.001699276537.6030162
GTACATG19950.036.9904371
TACATGG20050.036.571512
CCGAACT651.4102405E-436.1567461
ACATGGG20300.035.6458133
ATGGGTA3000.034.457625
GGCCTAT550.00271263434.184561
CATGGGG8000.034.0660554
ATAGACC702.1843905E-433.5626143
ATATATC1001.4092748E-632.8913653
CATGGGT4300.032.782094
AACGCAG10550.031.6220286
ATGGGAG3200.030.8356555
ATCAACG10750.030.5966173
TCAACGC11050.030.1911664
CAACGCA11100.030.055175
GTATAAC650.006144337428.9253961
TATATCT856.814269E-427.6398014
CAATATA1205.8199785E-627.4188691
Produced by FastQC (version 0.11.2)