FastQCFastQC Report
Sun 14 Apr 2019
SRR6576475_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576475_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences291804
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7420.2542802703184329No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4730.16209510493344848No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4440.15215692725253938No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4170.1429041411358309No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3790.12988170141601899No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3460.11857274060670861No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3450.11823004482460829No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3340.11446039122150484No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3330.11411769543940453No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3200.10966265027210044No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3120.10692108401529794No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3090.105892996668997No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3040.10417951775849542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8600.043.2350351
GTACATG18100.042.9054571
TGCGTAT555.2564257E-542.75057694
TACATGG19000.040.8100622
ACATGGG18800.040.244353
CATGGGA7750.036.381994
CATGGGT4400.036.3130874
AACGCAG10200.035.475416
TATAGGG801.15680505E-535.2450522
ATGGGAT2050.034.3854145
ATGGGAG3500.033.5667155
GTATAGG851.7335868E-533.2230531
ATCAACG11000.032.468173
CAACGCA11400.031.7411565
GATATAA600.004127493631.3773251
TATACTC753.2722935E-431.3289385
TCAACGC11400.031.3289344
ACGCAGA11850.030.5357977
GGTACCT2250.029.2603958
CATGGGG8350.028.7025574