FastQCFastQC Report
Sun 14 Apr 2019
SRR6576476_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576476_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418603
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8400.20066745818830728No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4250.10152817825003642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10000.041.831
ACGGGGT500.001700791537.694
GTACATG24450.035.5623741
TACATGG24750.035.5111122
ACATGGG24550.034.6517333
CATGGGT5250.033.123814
TCAACGC12550.032.2071724
CAACGCA12850.031.821015
AACGCAG13000.031.8153866
ATCAACG12850.031.4552523
ATGGGTA3650.029.616445
ATGGGGG3200.029.3749985
CATGGGG8750.029.0057144
GTGTACG650.006149759528.9230771
TCTAGAC1154.1880194E-628.6086963
GGTACCT3600.027.4166668
ACGCAGA15200.027.2105277
GAATACG459.5155113E-426.1111122-23
CGCAGAG15900.026.0125798
TATCAAC16000.025.556252
Produced by FastQC (version 0.11.2)