FastQCFastQC Report
Sun 14 Apr 2019
SRR6576477_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576477_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375638
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7460.1985954562637433No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4310.11473812553575517No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3960.10542064434375649No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9850.040.1070141
GTACATG21900.038.6551
TACATGG22650.037.1624372
ACATGGG22750.035.941653
ATCAACG11800.033.4524153
AACGCAG12050.032.364096
CAACGCA12100.032.2303545
TCAACGC12150.032.1019944
CATGGGA11800.031.8594444
CATGGGG9150.030.3012894
GGTACCT3200.029.3665128
ACGCAGA14100.027.6586727
TATCAAC14700.027.1907372
CGCAGAG14350.027.1768158
GTATAAT1606.847222E-826.4545141
CATGGGT5350.026.3510324
ATGGGAG5300.025.709555
ATGGGGA4800.025.4509815
TGGGTAC3900.025.3003836
ATGGGTA4100.025.2122275
Produced by FastQC (version 0.11.2)