FastQCFastQC Report
Sun 14 Apr 2019
SRR6576482_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576482_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293174
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9780.33359029108993293No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5690.19408269491837613No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA5240.1787334483958332No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5190.17702797655999508No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5070.17293484415398366No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC5060.17259374978681602No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4300.14667057788207685No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4210.14360072857756825No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC3990.13609665249988062No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3690.12586382148485198No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3670.12518163275051675No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3650.12449944401618152No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3650.12449944401618152No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3620.12347616091467867No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3510.11972412287583484No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC3380.11528989610265576No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3290.11222004679814718No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3170.10812691439213572No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3060.10437487635329191No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3010.10266940451745381No Hit
CTCCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCG2980.10164612141595093No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2940.10028174394728046No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCC200.002163874270.479418
GTATCAA8200.041.2562371
CTTAGGT906.1834544E-736.544883
GATAATA651.4133495E-436.1432842
GTACATG17550.035.6078261
TACATGG17500.034.9040872
ACATGGG17650.034.341243
ATAATAG550.00271761334.1718333
ATAATAC550.00271761334.1718333
GATATTA550.00271761334.1718336
GTACTAT550.00271761334.1718331
ATAGTAT550.00271761334.1718336
AACGCAG9650.034.0833056
ATCAACG9700.033.907623
CCATGCG851.753849E-533.166789
TCAACGC9950.033.0556644
TAGGTAT1001.4096313E-632.890395
CAACGCA10400.031.6253745
GTCTTAG1658.54925E-1131.3241831
ACCGAGT600.004161920431.3241798
Produced by FastQC (version 0.11.2)