FastQCFastQC Report
Sun 14 Apr 2019
SRR6576483_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576483_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190680
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3190.16729599328718273No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG156.893767E-493.998435
TACGGCG250.005229708756.3990555
CTATAAT500.001692051337.628981
TACATGG14350.036.383422
GTACATG14450.036.131631
TATACTG801.1537268E-535.2494135
GTATCAA5400.034.8416441
ATGGGAG2800.033.5708665
ACATGGG14600.033.478893
TGAACAG1309.104042E-932.5379145
GTATAGA600.004136726331.3574831
ATGGGGG2400.031.3328115
TGATACA600.004152700331.3328114
GTTATAG902.5829759E-531.3328083
CATGGGA7900.030.936194
CATGGGG5600.030.2137784
ATAAGAT650.006141979228.922593
AACGCAG7050.027.9995336
TCAACGC7050.027.3328784
CAACGCA7100.027.140395
Produced by FastQC (version 0.11.2)