FastQCFastQC Report
Sun 14 Apr 2019
SRR6576483_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576483_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190680
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3610.18932242500524438No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2080.1090832808894483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC504.5447814E-756.3974843
GTATCAA5250.043.1505781
AATAGAC853.9339466E-738.7041553
TACATGG13200.038.4528272
GTACATG13350.037.4739341
ACATGGG13550.037.1127363
AACGCAG6200.036.3854756
TATAGCC651.409443E-436.152232
ATCAACG6250.035.3424223
ATGGGAG3400.034.557285
CAACGCA6550.034.441215
TCAACGC6500.033.98314
CATGGGA8250.031.3319364
TATCAAC7250.030.4676062
GGGTACC1401.8742867E-830.2129387
GTATAGC804.6513428E-429.4974651
ACGCAGA7700.029.2973947
GGTACCT1452.636807E-829.1711128
CATGGGT3250.028.9217874
ATGGGCA1303.3083415E-728.9217835
Produced by FastQC (version 0.11.2)