FastQCFastQC Report
Sun 14 Apr 2019
SRR6576484_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576484_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181071
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3490.19274207355125889No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2280.1259174577927995No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1900.10493121482733293No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1830.10106532796527329No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6550.044.52061
AACGCAG7600.037.7195856
ATGGGGG1500.037.5959135
ATAAGAG500.001699150237.5959135
TACATGG12100.037.2852062
GTACATG12400.036.413311
ACATGGG12150.036.358193
GTCTAGA651.4028334E-436.179891
CAACGCA8000.035.8336035
TCAACGC8000.035.246174
ATCAACG8050.035.027253
TTATAAC550.002712440434.17813
CATGGGG4000.034.0712974
CATGGGT2350.033.9963044
CGCAGAG8500.033.7443858
ATGGGAT1554.1836756E-1133.3512155
ACGCAGA8700.032.950447
GTATAGG753.2509692E-431.3559061
GAGATCA753.2562454E-431.347249
GGGCGTT300.00415946631.32992632-33
Produced by FastQC (version 0.11.2)