FastQCFastQC Report
Sun 14 Apr 2019
SRR6576487_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576487_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432884
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13380.3090897330462664No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7790.17995583112334945No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6510.15038670867946147No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5910.13652618253388898No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5420.1252067528483381No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5370.12405170900287375No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4900.11319429685550864No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4890.11296328808641576No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4880.11273227931732288No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4730.10926714778092977No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4480.10349192855360789No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4370.10095083209358627No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGCG156.9000566E-493.9973455
GTACATG24000.037.8078651
TACATGG24400.037.380752
GGGTACC5050.037.2266737
GTATCAA10150.037.056131
GGTACCT5200.036.1528248
TGGGTAC5300.035.47076
ACATGGG25500.035.202933
ATGGGTA5350.035.139195
GTCCATA702.1786834E-433.582121
CATGGGG12950.031.9373234
AACGCAG11750.031.199126
ATGGGGG4700.029.9991535
TCAACGC12300.029.8040354
CAACGCA12350.029.683375
CATGGGT7350.029.4141354
TATTACT650.00614042828.9322852
ATCAACG12800.028.6398163
CTATTAG700.00881634826.8656941
ATAGACC700.00883126526.8563823
Produced by FastQC (version 0.11.2)