FastQCFastQC Report
Sun 14 Apr 2019
SRR6576487_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576487_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432884
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11300.2610399090749485No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7090.1637852172868482No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6460.1492316648339971No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5570.12867188438473123No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5480.12659280546289536No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5480.12659280546289536No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4960.11458034947006589No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4690.10834311270455828No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4690.10834311270455828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9600.037.8090631
GTATAAA1001.3764384E-632.9969981
GTACATG22850.032.5947231
TACATGG23400.031.7366812
ACATGGG23250.031.5371113
TATGGGT804.774572E-429.3765184
GTATAAT650.00606214929.0083521
ATGGGTA4100.028.6600175
CATGGGG11900.028.4384444
ATCAACG12950.027.9473883
CAACGCA13150.027.8797665
TCAACGC13100.027.6273784
AACGCAG13550.027.0567476
TATACAG1606.891605E-826.4388665
TATATAC909.5051964E-426.112463
GTATAAG950.001281097324.8097721
CTTAGAC950.001302880524.7381173
GGGTACC4650.024.2565167
ATATACA1802.153356E-723.5012134
GGTACCT4600.023.4984998