FastQCFastQC Report
Sun 14 Apr 2019
SRR6576488_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576488_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381985
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12170.3185988978624815No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7100.1858711729518175No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6120.1602157152767779No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5770.1510530518214066No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5550.14529366336374466No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5330.1395342749060827No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4690.1227796903019752No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4370.11440239799992147No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT4330.11335523646216475No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4270.11178449415552966No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4270.11178449415552966No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG4150.10864300954225951No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4040.10576331531342853No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG4020.10523973454455017No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4010.10497794416011101No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3950.10340720185347593No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3880.10157466916240167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8850.038.2468261
TATATAG500.001701803637.594615
TGGGTAC4150.037.3681346
TACATGG20850.036.531882
GGGTACC4250.036.4888847
GTACATG21450.036.1628651
ATGGGTA4500.034.461725
ACATGGG21900.034.1183933
CATGGGT6250.033.8351484
TATTATC753.264187E-431.3452532
GTAGACC902.5919657E-531.3288384
GTAGGGG1052.0650114E-631.3247389
ACGTAGG902.5942863E-531.32473894
GGTACCT5150.030.1121868
AACGCAG11400.029.6799536
ATCAACG11350.029.3966643
CAACGCA11500.029.013235
CCTAGCC650.00615340428.918933
TCAACGC11600.028.3584
CACTCTA3350.028.0520049
Produced by FastQC (version 0.11.2)