FastQCFastQC Report
Sun 14 Apr 2019
SRR6576489_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576489_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357478
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7390.20672600831379834No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4770.1334347847979456No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4130.1155315851604854No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3940.1102165727681144No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3710.10378261039840214No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3690.1032231354097315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8600.040.9933781
GTACATG23150.039.7975121
ACATGGG23650.038.1557663
TACATGG24400.037.951352
ATGGGTA3800.032.1573225
ATCAACG10700.032.064853
CATGGGT5800.031.6028844
CATGGGA13100.031.5719554
GGTACCT3150.031.3327758
CATGGGG7600.030.92054
GGGTACC3200.030.84327
CAACGCA11100.030.4859455
TCAACGC11150.030.3492364
AACGCAG11450.029.964536
TATACTT1154.1863514E-628.6081835
ATGGGAG5400.027.8513555
ATGGGAT4400.027.7722325
TTAATAC2104.9112714E-1126.8566653
CGCAGAG13450.025.5088398
GTACCTG3950.024.9868979
Produced by FastQC (version 0.11.2)