FastQCFastQC Report
Sun 14 Apr 2019
SRR6576489_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576489_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357478
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7900.22099262052489946No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5120.14322559709968166No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4370.12224528502453298No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4360.12196554753019767No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4040.11301394771146756No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3820.1068597228360906No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3780.10574077285874935No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3660.10238392292672557No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7950.043.2984771
GTACATG20750.041.813511
ACATGGG20950.041.505433
TACATGG21450.040.3188172
CATGGGA12500.035.7215964
CAACGCA9400.035.001565
ATCAACG9600.034.761973
TCAACGC9500.034.6331254
AACGCAG9350.034.1833426
GGTACCT2700.033.0709238
ATGGGTA3750.032.5881235
GGGTACC3000.031.330357
CATGGGG6400.030.8451294
ATGGGAT5200.030.732145
CATGGGT5900.028.6792474
ATGGGAC2151.8189894E-1228.4198725
ACGCAGA11250.027.9884457
GTCTTAG1354.489484E-727.9430081
CGCAGAG11450.027.0891238
TATCAAC12700.027.0169542
Produced by FastQC (version 0.11.2)