FastQCFastQC Report
Sun 14 Apr 2019
SRR6576490_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576490_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324338
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7730.23833161701681582No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4700.1449105562715439No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4200.12949453964691157No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4150.12795293798444832No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3960.12209485166708804No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3880.11962828900714687No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3580.11037867903236746No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3510.10822043670491895No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3450.10637051470996307No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3340.10297899105254395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7350.046.702651
GTACATG20000.041.144711
TACATGG20700.039.7533452
ACATGGG20800.039.3058323
ATGGGTA3000.037.5890245
CGCGGCA250.001703922837.5890236-37
ATCAACG9350.036.181953
CATGGGT5100.035.0093844
AACGCAG9950.034.944576
CAACGCA9900.034.171845
TCAACGC9950.034.0001224
GTATAGT851.7451817E-533.192371
CATGGGA11650.032.2652554
GGTACCT2650.031.9152078
ACCCTTG1658.54925E-1131.3241847
GGGTACC2750.030.7546547
ACGCAGA11700.029.7178177
CATGGGC3350.029.4540844
GTGCTAA804.7600246E-429.389081
CGCAGAG12000.029.3664268