FastQCFastQC Report
Sun 14 Apr 2019
SRR6576490_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576490_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324338
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7840.24172314067423492No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4530.1396691106191689No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4350.13411934463430125No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4120.12702797698697038No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4100.1264113363219851No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3800.11716172634720569No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3780.11654508568222041No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3700.11407852302227922No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3620.11161196036233804No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3290.10143738939008072No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG250.005238916356.3829774
GTATCAA9800.047.0656361
TCAACGC12400.036.3761144
AACGCAG12600.036.171626
GTACATG22400.035.9295731
ATCAACG12750.035.3775563
TACATGG23050.034.862532
CAACGCA13100.034.4323545
ACATGGG23200.034.2267383
GTTTAGA600.00412831531.3770921
ACGGCGC300.00416580631.32387722-23
CGCAGAG14700.031.0281668
ATGGGTA4250.030.9553625
ACGCAGA14900.030.5927967
CATGGGT6300.029.8322644
TATCAAC15750.029.2401242
CATGGGG7500.028.8179684
GGTACCT3450.028.6221268
ATGGGAC2350.027.9915495
CATGGGC3100.027.2820854