FastQCFastQC Report
Sun 14 Apr 2019
SRR6576491_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576491_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences653367
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14080.2154990992811085No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8280.12672816349769733No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7850.12014686998272027No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7710.1180041232569138No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6680.10223962948848044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14250.041.044991
GTACATG34250.037.3217541
ACATGGG34200.036.5519523
TACATGG34600.036.273542
TGGGTAC7100.034.421786
GGGTACC6750.033.4214867
CATGGGG18850.032.662414
GGTACCT6850.032.2474678
ATGGGTA7650.031.947015
ATCAACG18500.030.7375133
CATGGGT10900.030.6140044
TCAACGC18800.030.499334
CAACGCA19300.029.7091945
AACGCAG19600.029.4942476
TAGATCG700.00883422726.8565525
TATATAC2500.026.3174083
ATGGGGG8400.026.2970395
TATACAG2750.025.63585
GTACCTG8850.025.4909659
TATCAAC22900.025.247852
Produced by FastQC (version 0.11.2)