FastQCFastQC Report
Sun 14 Apr 2019
SRR6576492_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576492_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences393663
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7060.17934121316964002No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4710.11964548357351339No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4290.10897645956058863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10500.042.554631
CCGAACT500.001694625537.6272541
ATCAACG12150.035.973953
TCAACGC12500.034.9666754
CAACGCA12600.034.3161665
AACGCAG12800.033.7799726
CTAGTAC1501.0659278E-931.3321463
TACATGG19800.030.386552
AAAGGCG1102.9627881E-629.90795994
TATCAAC14850.029.4369682
GTACATG20500.029.3676131
ACATGGG20500.028.657453
ACGCAGA15600.027.71697
CGCAGAG15750.027.452938
CATGGGG8300.027.1796954
GTACTTG1406.3103835E-726.876611
GTATACG700.00879794526.876611
AAGACCG700.00883072326.8561255
CCGCGCA700.00883072326.8561259
GCAGAGT17150.025.4859189
Produced by FastQC (version 0.11.2)