FastQCFastQC Report
Sun 14 Apr 2019
SRR6576492_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576492_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences393663
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6620.16816414039419503No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6190.15724109200001016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10450.037.894981
GTATAGC1554.0017767E-1133.4564321
TACATGG21250.030.525862
ACATGGG21500.029.7260953
CAACGCA13200.029.552675
AACGCAG13250.029.441156
TCAACGC13250.029.437414
GTACATG22750.029.21841
ATCAACG13450.028.9996813
TAGCCCT3300.028.4808854
TCCGACC1406.351129E-726.8568158
CGGTAAC350.00883488526.85681586-87
ATAGCCC1956.039045E-1026.5091323
GTATAAG1257.819463E-626.4001711
CATGGGT5850.025.7058244
TACTAGG950.001302190124.7396852
TATCAAC16150.024.4486312
GTATAAT1351.4204048E-524.44461
CATGGGG8150.023.640884
ACGCAGA16500.023.3572927
Produced by FastQC (version 0.11.2)