FastQCFastQC Report
Sun 14 Apr 2019
SRR6576493_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576493_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304491
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6040.19836382684545684No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3250.10673550285558522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6800.042.2716641
GTACATG15300.038.1907921
TACATGG15700.035.922562
ACATGGG15600.035.8515623
ATGGGTA2600.034.3451925
CATGGGT4250.034.2813954
ATCAACG8800.033.6467863
AACGCAG9500.031.6622756
TCAACGC9400.031.4991174
CAACGCA9550.031.4965025
CATGGGG6600.030.6203564
CTCTATA856.805615E-427.6462862
ACGCAGA11000.027.3446887
CGCAGAG11200.026.8563928
TATCAAC10750.026.2318252
TGGGTAC3050.026.1959926
GTAACAC909.503108E-426.1103823
GGGTACC2900.025.930317
GGTATCA3850.024.4792251
GCAGAGT12150.024.369699
Produced by FastQC (version 0.11.2)