FastQCFastQC Report
Sun 14 Apr 2019
SRR6576494_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576494_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences276368
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6380.23085161813234528No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3550.12845191918022347No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2910.10529439008857755No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2880.10420888091240664No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCTA301.3710758E-462.6505053
CCGCTAA405.6764076E-446.9878734
ACCTAGC450.001013904141.7670026
GTATCAA7350.041.5840031
TGCCCGC853.9267616E-738.7239231
GATATAC500.001695902637.617531
CGCTAAA500.00170194637.59035
CATGGGT3950.034.497434
GTACTAG550.002707258634.197751
GTACATG17100.032.997831
TACATGG16700.032.9434932
AACGCAG9500.032.6442076
ATCAACG9450.032.3197023
ACATGGG16750.032.2603343
TCAACGC9550.031.981284
GCTATAC600.00416081431.3252533
GTTGTAC600.00416081431.3252533
CAACGCA9800.031.165435
GTATATC804.7585895E-429.388691
GTATAGG650.006132208328.9365581