FastQCFastQC Report
Sun 14 Apr 2019
SRR6576494_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576494_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences276368
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5680.20552307068835754No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3110.1125311179297169No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2920.10565622648063451No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2860.10348520812829272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7000.042.3663141
GTACATG16300.037.254631
TACATGG16400.036.68062
ACATGGG16350.036.217883
AACGCAG8100.035.9665156
CAACGCA8550.034.0797045
GTTATTC1405.0749804E-1033.5692983
ATCAACG8700.033.4921233
TCAACGC8700.033.4921234
CATGGGG7150.030.893144
CGCAGAG9900.029.4484628
ACGCAGA9950.029.2792727
CATGGGT3600.028.7203984
TATCAAC10400.028.4693452
CATGGGA7500.026.9449564
GTATAGG909.4128307E-426.1520461
CCCTAAG909.5028494E-426.1094512
ATACTCT909.5128955E-426.1047296
ATTATAG950.00130256224.7352713
GCAGAGT12100.024.4828119
Produced by FastQC (version 0.11.2)