FastQCFastQC Report
Sun 14 Apr 2019
SRR6576495_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576495_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences376109
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5320.14144835672637454No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4100.1090109516124315No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAT250.001702209137.597512-13
ATCACGC250.001702209137.597522-23
GTACATG21400.035.7004781
TACATGG21100.035.6469152
ACATGGG21250.034.952853
GTATCAA10100.031.284281
TAGGGTC953.7439404E-529.6861844
CATGGGG7650.028.2633174
CATGGGA12200.028.1248234
CGCGATC350.00883697826.8553614-15
ATGGGAC3000.026.6351035
CATGGGT4950.026.5876274
GGTACCT3200.024.9670938
AACGCAG12200.024.2721066
ATCAACG12950.023.595443
TCAACGC12750.023.5937274
ATGGGTA3600.023.5015625
GGGTACC3200.023.498447
CAACGCA13050.022.6911625
TAAGACC1050.002331697122.3824394
Produced by FastQC (version 0.11.2)