FastQCFastQC Report
Sun 14 Apr 2019
SRR6576497_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576497_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437468
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9650.2205875629760348No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6210.14195324000841203No Hit
CCTATAAGCAGTTCTTGTATTTTTATTCACACAGGTCTGATTAAGATGAA5450.1245805407481233No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5430.1241233644517999No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5260.12023736593305111No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4960.11337972148820027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8200.051.1208571
GGGCGTA652.781835E-643.382619
ACATGGG24000.039.1693343
GTACATG24550.039.1381841
TACATGG25300.037.8998152
AACGCAG11400.035.4585536
TCAACGC11150.035.4104844
CAACGCA11450.034.8932045
ATCAACG11400.034.6339343
CATGGGA9250.034.5537034
CATATAG600.004156804631.3354663
CATGGGG12100.031.0764984
ACGCAGA13250.030.5042517
ATGGGTA4200.030.2163435
GCGATAA404.781255E-429.3736478-79
ATGGGAG5500.029.0565225
CGTACAT650.00614801128.9250475
CGCAGAG13650.028.9217418
GTATAAA1503.6114216E-828.2600691
TATCAAC14200.028.1357172
Produced by FastQC (version 0.11.2)