FastQCFastQC Report
Sun 14 Apr 2019
SRR6576498_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576498_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences396558
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10680.2693174768886266No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6230.15710186151836553No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5290.1333978888334115No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5230.13188486930032933No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5210.1313805294559686No Hit
CCTATAAGCAGTTCTTGTATTTTTATTCACACAGGTCTGATTAAGATGAA4650.11725901381386833No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4260.10742438684883421No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4200.10591136731575204No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4010.10112013879432517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8350.045.0279461
GTACATG23500.041.598161
TACATGG24050.040.6468472
ACATGGG23450.040.273773
TCGTTGA500.001703161637.5888569
AACGCAG10600.036.7909776
ATCAACG10350.036.3177343
CATAATG651.412188E-436.1522452
GGGCTAG651.414285E-436.143136
TCAACGC10550.035.6292464
CAACGCA10950.035.1859135
CATGGGA9250.034.5411074
GCGTCCG550.002718822334.1716849
ATGGGAG5550.033.863835
CATGGGG11300.033.680284
ATGGGTA3700.033.0172355
GGGTACC3850.031.7308527
GATATAG600.00415862631.3319471
GTCTTAC600.00415862631.3319471
ACGCAGA12650.030.8288047