FastQCFastQC Report
Sun 14 Apr 2019
SRR6576498_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576498_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences396558
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9190.23174415848375268No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5450.1374326075882973No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5270.13289354898905079No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5100.12860666031198464No Hit
CCTATAAGCAGTTCTTGTATTTTTATTCACACAGGTCTGATTAAGATGAA4760.12003288295785232No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4540.11448514466988437No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4060.10238098840522698No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3970.10011145910560372No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAT156.8824936E-494.0561458
CCGTATG250.00522116156.4336939
TACCGTA250.00523421256.398117
GTATCAA9700.047.069651
GTACCGT405.673134E-446.998426
GTACATG24600.040.372751
GCCTAGT704.5806974E-640.3454171
ACATGGG23500.040.1935843
TACATGG24750.039.8724252
CATGGGG11900.038.304814
ATCAACG12000.037.2023933
TCAACGC11950.036.9648134
AACGCAG12150.035.9741066
CAACGCA12650.034.9193275
ATGGGTA3450.034.0525325
GTACTAA702.1635278E-433.621181
TATCAAC14250.032.3176462
ACGCAGA14250.031.002477
CGCAGAG14600.029.956248
GTATCAC804.7340666E-429.4185311