FastQCFastQC Report
Sun 14 Apr 2019
SRR6576499_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576499_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304722
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5310.17425719180105145No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3290.10796726196336333No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3210.10534191820741527No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8500.044.786361
TACATGG19850.037.409012
GTACATG20400.037.3219681
ATCAACG10550.035.1928373
ACATGGG20650.035.0493773
CAACGCA11100.033.8724565
TCAACGC11000.033.753134
GTATTAC702.1817876E-433.5700231
AACGCAG11250.033.0030636
TATCAAC11600.032.817592
CATGGGA11400.032.5688134
ATGGGAG5100.029.4889625
ACGCAGA13300.027.9161247
CGCAGAG13550.027.7479158
GATCGTC856.806183E-427.64597
GCAGAGT14350.026.5285059
ATGGGAC2156.548362E-1126.2314595
CATGGGG7050.025.998914
GTATTAA1151.3743578E-424.5207121
CTGATCG1151.3743578E-424.5207125
Produced by FastQC (version 0.11.2)