FastQCFastQC Report
Sun 14 Apr 2019
SRR6576499_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576499_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304722
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6210.20379230905546694No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3520.11551512526171395No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3430.11256161353627242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7750.049.894631
CAACGCA9400.040.008035
ATCAACG9600.039.174533
AACGCAG9600.039.174536
TCAACGC9800.038.854744
TACATGG19750.037.607552
ACATGGG18950.037.4587063
GTACATG20100.037.3029141
ATAGGAC1107.651215E-834.1886833
ACGCAGA11250.033.8356867
CATGGGG6800.033.1831324
TATCAAC11400.032.989082
CGCAGAG11700.032.935978
CATGGGA10350.032.7022174
TATACAG1102.954388E-629.9150965
GTATAAG953.659322E-529.783061
GTATATA1652.8430804E-928.5797061
GCAGAGT13600.027.989089
ATGGGAT3800.027.2159885
CGTGCCC700.00881737626.8625373
Produced by FastQC (version 0.11.2)