FastQCFastQC Report
Sun 14 Apr 2019
SRR6576500_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576500_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences276548
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6180.22346934347744332No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3330.12041309284464179No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2990.10811866294458829No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2810.10160984711514817No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCTA255.5598615E-575.17833
TATACCG250.00523782756.3837245
CCGCTAA405.6772714E-446.9864354
GTATCAA7450.043.517641
TGCCCGC555.2816387E-542.7149431
TACATGG17100.039.017982
GTACATG18000.038.3722571
ACATGGG17400.036.9950683
ATCAACG9050.035.8239143
AACGCAG9100.035.627086
TCAACGC9200.035.2398264
CAACGCA9350.034.6744845
CGCTAAA550.002717334.1719555
CTAGTAG600.004161442631.3242913
CATGGGT3500.030.87684
CATGGGA9450.030.3298684
ATGGGTA2200.029.900465
TATCAAC11000.029.473312
CCTATAC804.7796764E-429.3665223
CGCAGAG11250.028.818358
Produced by FastQC (version 0.11.2)