FastQCFastQC Report
Sun 14 Apr 2019
SRR6576501_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576501_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102617
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3250.31671165596343687No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2040.1987974702047419No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2040.1987974702047419No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1860.1812565169513823No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1740.16956254811580926No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG1590.15494508707134294No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC1510.14714910784762758No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA1510.14714910784762758No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1470.14325111823576991No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT1460.14227662083280548No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1320.1286336571913036No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1320.1286336571913036No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG1280.1247356675794459No Hit
CTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCG1120.10914370913201515No Hit
CTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGACACTCAGCTA1100.10719471432608632No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT1090.1062202169231219No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT1060.10329672471422863No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG1050.10232222731126421No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG1040.1013477299082998No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAATGC200.00215775370.4967048
CCTATTG405.653742E-446.99780394
CATGGGT1700.038.704084
ATGGGAG1357.2759576E-1238.294515
GTACATG9250.037.6165771
ACATGGG9250.036.0739943
TACATGG9550.035.942542
GTAGAGA550.002699697934.1968881
ACCCTTG550.00270616434.180227
GGGTACC851.7398816E-533.1749237
ATGGGTA1151.12069756E-732.6941265
CATGGGA5200.031.633144
CATGGGG3400.030.4103474
TCTGGGA804.7386863E-429.3879492
ATGGGGC1303.283276E-728.9217285
AACCCTT650.00612983528.9217286
GAATATA700.008780329526.8689841
GGCTTAC700.008780329526.8689841
GGGAGAT700.00880124526.8558927
CGCAGAC350.00882142926.85589256-57
Produced by FastQC (version 0.11.2)