FastQCFastQC Report
Sun 14 Apr 2019
SRR6576501_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576501_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102617
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3270.31866065076936567No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2020.19684847539881306No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1870.18223101435434674No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1670.16274106629505833No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1590.15494508707134294No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG1500.14617461044466315No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1500.14617461044466315No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1470.14325111823576991No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC1430.13935312862391222No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1430.13935312862391222No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA1320.1286336571913036No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1280.1247356675794459No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG1280.1247356675794459No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT1130.11011820653497958No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC1100.10719471432608632No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG1090.1062202169231219No Hit
GGGCGGGGACGGGCGGTGACTCGCCTCGCGGCGGACCGCCCGCCCGCTCC1080.10524571952015749No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT1060.10329672471422863No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACATA255.523331E-575.2327652
GTATAAG504.448484E-756.5348321
TATAAGG555.235903E-542.7458882
ATGGGAT952.0767402E-839.596195
GTCCTAG757.257564E-637.6898881
TCCTAGG500.001691187137.6163832
CCTATTG500.00169524537.5980594
TATAAGA550.002699766334.196712
GTACTTT4000.032.9786531
CATGGGT1605.820766E-1132.3265764
GTACATG8550.031.959261
TACATGG8800.031.5250932
ACAATAC753.2466388E-431.3469873
ATGGGGG902.5634974E-531.3469855
ACCCTTG600.004144555431.3317097
GAGACGC300.004154146631.33170932-33
ACTTTTT4100.030.9647053
CATGGGG2750.030.777044
TACTTTT4150.030.5916372
ACATGGG8650.030.4410043