FastQCFastQC Report
Sun 14 Apr 2019
SRR6576502_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576502_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94473
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG2800.2963809765753178No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2050.2169932149926434No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1880.19899865570057054No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1840.19476464174949457No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1770.18735511733511162No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1710.18100409640849768No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1410.1492489917754279No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG1360.14395647433658293No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1360.14395647433658293No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC1350.14289797084881395No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1320.13972246038550698No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA1220.12913742550781704No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT1220.12913742550781704No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC1200.12702041853227905No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG1180.12490341155674109No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1170.1238449080689721No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG1110.11749388714235812No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA1060.11220136970351317No Hit
ACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAG990.10479184528913023No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG970.10267483831359224No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGCC200.002158170670.4888843
GGGTACC857.8089215E-944.2283177
ATGGGTA901.2936653E-841.771195
CATGGGT1750.037.594074
ATGGGAG1055.034599E-835.803885
GTACATG7500.035.7901541
ACATGGG7650.035.0140843
TACATGG7850.034.720642
GGTACCT959.302039E-734.644468
CATGGGC959.3407107E-734.6261184
GTCATAG550.002678539434.248951
GTCCTAG702.1450188E-433.6373631
ATGGGTG702.1719256E-433.5661355
ACACTCT851.733442E-533.188818
AACACTC851.739744E-533.1712387
TGGGTAC1151.12018824E-732.6904956
AACGCAG5250.031.3283946
GTACTTT5300.031.0986921
ACTTTTT5450.030.1787283
TACTTTT5650.029.110452
Produced by FastQC (version 0.11.2)