FastQCFastQC Report
Sun 14 Apr 2019
SRR6576503_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576503_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314551
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8540.27149810364614957No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5300.1684941392651748No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4430.14083566734806122No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4060.12907286894653014No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3950.12557582077310198No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3910.12430416689185536No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3560.1131771954309476No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3550.11285928196063594No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3480.1106338876684544No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3170.10077857008879323No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3170.10077857008879323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6550.054.5857621
CGCTAAA451.6113621E-552.2216035
CCCGCTA451.6113621E-552.2216033
GCAAACG502.9877137E-547.0443151
ATCAACG8800.037.9200063
GTCGTGA500.001692294837.6354521
TCAACGC8950.037.2844734
CAACGCA9050.036.8724945
CCGCTAA651.4112552E-436.153424
AACGCAG9600.035.2495846
GTACATG15700.034.7588541
TACATGG15650.034.541822
ACATGGG16100.034.4467963
GTATACT550.00270150834.2140461
TAGTACA550.002714180234.1814164
GGTATCA3350.033.703391
GGGTACC2800.031.8924797
GGTACCT2950.031.8640298
GCGTTAT902.5713665E-531.3628731
TGGGTAC2850.031.3329646
Produced by FastQC (version 0.11.2)