FastQCFastQC Report
Sun 14 Apr 2019
SRR6576504_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576504_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121330
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4510.371713508612874No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2660.21923679221956646No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2420.19945602901178605No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2370.1953350366768318No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2300.18956564740789583No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2280.1879172504739141No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2220.182972059671969No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2010.16566389186516114No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT1980.1631912964641886No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC1840.15165251792631665No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG1720.14176213632242646No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG1720.14176213632242646No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG1710.1409379378554356No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG1710.1409379378554356No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC1590.13104755625154538No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG1490.12280557158163687No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT1480.12198137311464602No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC1460.12033297618066431No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT1420.1170361823127009No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT1370.11291518997774665No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG1340.11044259457677408No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG1310.10796999917580152No Hit
GAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAA1300.10714580070881068No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG1300.10714580070881068No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC1300.10714580070881068No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT1240.10220060990686558No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG1220.10055221297288389No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGCG250.005225695756.3975265
GCAAACG250.005225695756.3975261
GAAGTCG250.005225695756.3975266
ATAGCAC405.6577695E-446.9979363
CATGGGT1850.040.6468664
CAATCCG308.805781E-539.16494890-91
ACATGGG11250.038.851633
GTACATG11300.038.6797181
CATGGGG3350.037.8789374
TACATGG11450.037.762542
TATTTCG500.001696398637.598355
CGTAAAT250.001699748237.5983534-35
TAAATCT500.001696398637.5983594
ATACATA500.001696398637.598353
CTGGACT500.001696398637.598354
GGAGTCT651.4064141E-436.152266
GTATCAA7050.035.998421
ATATAGA1055.0498784E-835.8079531
ATGGGTA1204.143658E-935.248455
CATGGGC2400.035.248454