FastQCFastQC Report
Sun 14 Apr 2019
SRR6576504_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576504_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121330
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4130.3403939668672217No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2730.22500618148850243No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2650.2184125937525756No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2600.21429160141762135No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2500.20604961674771283No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2340.19286244127585925No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2000.16483969339817028No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT1980.1631912964641886No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG1810.1491799225253441No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1750.144234731723399No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG1750.144234731723399No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG1740.14341053325640815No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC1670.13764114398747218No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG1610.13269595318552707No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC1600.1318717547185362No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1590.13104755625154538No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG1580.13022335778455452No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1390.11456358691172834No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT1360.11209099151075579No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG1310.10796999917580152No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG1310.10796999917580152No Hit
GGGCGGGGACGGGCGGTGACTCGCCTCGCGGCGGACCGCCCGCCCGCTCC1300.10714580070881068No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG1250.10302480837385641No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGCG156.892014E-493.983515
TCAGAGT200.002159807870.487636
TGCGTAT250.00521988856.4133694
CTGTGCG250.005228417556.3901069
GGGTCCT352.9295625E-453.704867
ATGGGTA1700.046.991765
GGGTACC1250.045.1120877
CATGGGT2750.042.719784
GGTACCT1400.040.278658
GTATCAA6650.038.945631
TATCATG500.001697495137.5934032
GTACATG11500.036.4426421
TGGGTAC1551.8189894E-1236.3807146
ACATGGG11050.036.1475073
CATGGGG3150.035.803244
TACATGG11650.035.495922
ACTGTGC550.002709812734.175828
ATGGGAT1001.3993395E-632.894235
GTCCTGG600.004108293431.3925381
GAATAGG1052.0153584E-631.3925381
Produced by FastQC (version 0.11.2)