FastQCFastQC Report
Sun 14 Apr 2019
SRR6576506_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576506_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343266
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7190.20945855400767918No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4830.14070720665606265No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4280.12468464689191472No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4280.12468464689191472No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3910.11390583395966976No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3540.1031270210274248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13150.039.314131
GTACATG24650.036.797821
ACATGGG24500.036.0639653
TACATGG25400.035.89632
AACGCAG15150.033.8139236
ATGGGCG702.1823005E-433.570185
ATCAACG15600.033.1397933
CAACGCA15650.033.0339165
TCAACGC15650.033.0339164
GTATAGT1850.033.02581
TCCGTAC600.004157711331.3321673
ACGCAGA16900.030.3124837
CGCAGAG17250.029.9698988
CATGGGA13800.029.6293334
ATGGGAG4400.028.8398365
CATGGGG8300.028.3121994
TATCAAC18550.028.1229442
ATGGGAT3650.027.040095
GTAAGAC700.00882914926.8561443
GTAATAC1057.4085554E-526.8561423
Produced by FastQC (version 0.11.2)