FastQCFastQC Report
Sun 14 Apr 2019
SRR6576506_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576506_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343266
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6370.18557037399567683No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5080.14799018836703898No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4110.11973221932845082No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3630.10574889444337629No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3470.10108778614835141No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10800.046.2519231
ATCAACG13400.036.823793
AACGCAG13700.036.0174336
TCAACGC13450.035.9881024
GTACATG21050.035.5953031
CAACGCA13650.035.4608045
TACATGG21600.034.5929342
ACATGGG21400.034.477033
TATCAAC15450.031.9377882
ACGCAGA15600.031.9319387
GTATGAC600.00415947731.329453
CGCAGAG16300.030.5606298
ATGGGCG650.006151963528.919495
CATGGGA12500.028.5724564
CATGGGG7800.027.7145124
GCAGAGT17950.027.489639
GTATAGG1351.4240521E-524.4349771
ATGGGAG5400.024.367355
TAGCCCT1951.7496859E-824.0995754
CATGGGT4550.023.7552974
Produced by FastQC (version 0.11.2)