FastQCFastQC Report
Sun 14 Apr 2019
SRR6576507_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576507_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318031
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6970.21916102518307964No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4810.15124311780927016No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4220.13269146718401667No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3810.11979964217324728No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3690.11602642509692453No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3320.10439233911159604No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3240.10187686106071421No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3240.10187686106071421No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10350.039.515311
AGGGCCG608.82359E-539.156185
GTACATG23050.036.710311
TACATGG23450.036.0841262
ACATGGG23500.035.9903643
CATGGGG6600.034.172674
CACCGTC702.1848953E-433.5624437
ATGGGAG4700.032.9911655
GTCCTAC753.2663418E-431.339731
CACTACT753.2663418E-431.339732
ACCGTGC600.004161947431.3249458
CATGGGA13550.029.8222754
AACGCAG13650.029.2595656
TCAACGC13600.029.0216454
GAGGTAC650.00614143528.9289821
TAATAAC650.00615561128.9153353
ATCAACG13650.028.9153353
CAACGCA13950.028.29355
CATGGGT6450.027.682514
TCTAATA700.008817797526.8626272