FastQCFastQC Report
Sun 14 Apr 2019
SRR6576508_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576508_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184973
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG2460.13299238267206565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA2850.051.2324331
CAACGCA4250.036.493285
ATCAACG4450.034.853133
AACGCAG4600.034.738336
CTAACAC702.1782439E-433.5706523
TCAACGC4700.032.999244
ACATGGG8400.032.4516373
GGCGTTA300.004157835631.33261358-59
TACATGG8500.030.9639952
GTACATG9200.030.2059361
TATCAAC5250.029.5421772
TATAGTG804.7662083E-429.3743255
ATAACTC650.006141696628.9224133
ACGCAGA5450.028.458067
CATGGGT2350.027.9993554
ATACTGT1057.3896896E-526.8565256
CATGGGA4750.026.7151744
CGCAGAG5800.025.9304398
ATGGGAT1859.924406E-925.4048215
GTACAGA1301.0635744E-525.3619631
Produced by FastQC (version 0.11.2)