FastQCFastQC Report
Sun 14 Apr 2019
SRR6576509_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576509_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150342
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG1730.11507097151827168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA2600.045.1799931
GCCTAAT450.001012498641.7663961
TACATGG6800.041.4730832
GTACATG6850.040.470721
ATCAACG3050.040.0679863
TCAACGC3200.038.1897964
ACATGGG7400.038.1104053
CATGGGG2850.037.9321064
CAACGCA3350.037.8828775
TAAGAGT550.002708733334.1838766
AACGCAG3800.033.3967486
ATGGGGA1703.6379788E-1233.1784675
ATGGGGG1309.069481E-932.5404175
CATGGGA3950.032.1285174
CTATGAC600.00414836331.335223
ATACCGT600.00414836331.335226
TATCAAC3950.029.7486272
TCTATGG650.00613558528.9248163
GTGTTAA650.006145572328.9151951
ACGCAGA4500.028.2016987
Produced by FastQC (version 0.11.2)