FastQCFastQC Report
Sun 14 Apr 2019
SRR6576513_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576513_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167955
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5080.3024619689797863No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3230.19231341728439164No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2830.16849751421511716No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2820.16790211663838528No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2690.16016194814087106No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2580.15361257479682058No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2480.14765859902950196No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2410.14349081599237892No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2330.138727635378524No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2020.12027031049983627No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA1850.11014855169539459No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG1830.10895775654193088No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG1830.10895775654193088No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG1810.10776696138846714No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG1700.10121758804441665No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAACG250.005229131356.3976751
GCGAACG250.005235299556.38088294
TGCGTAT250.005235299556.38088294
CTAGTAC405.672274E-446.984073
AACAGGG751.4748184E-743.8518037
GTATCAA8300.042.4681321
GATTGGC500.001700722337.5872579
TAGTACC500.001700722337.5872574
ATGGGAG2900.037.2632335
CATGGGT2300.034.727364
GTATAGG702.1776228E-433.5700451
GTACATG15000.033.2119641
ATCAACG10400.032.9792023
AACGCAG10350.032.684576
ACATGGG14950.032.3702963
TACATGG15350.032.1485142
TCAACGC10900.031.8974444
CAACGCA10800.031.7577515
ACGCAGA11500.029.4161157
CTCGTCC650.00614959628.9132738
Produced by FastQC (version 0.11.2)