FastQCFastQC Report
Sun 14 Apr 2019
SRR6576514_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576514_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437432
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6050.13830721117796596No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5580.1275626840286033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9050.045.748061
ATCAACG10550.038.7577253
TCAACGC10600.038.5749054
GTACATG20200.037.2655141
CAACGCA11100.036.83735
ACATGGG20250.036.206883
TACATGG20750.035.7997062
CATGGGG12100.035.7350164
AACGCAG11850.034.1092076
TATCAAC13600.030.4217532
GTAGTAC804.7765058E-429.374573
ATGGGTA3050.029.2782615
ACGCAGA14350.028.1668347
CGCAGAG14450.027.971918
ACGTAAG350.00883551926.8567516-17
ATGGGGA6700.026.6563285
CTAGTAC1101.0170249E-425.635993
CATGGGT4800.025.4579624
AGGTCGC950.00130340524.7364836
GGTACCT3500.024.1710768
Produced by FastQC (version 0.11.2)