FastQCFastQC Report
Sun 14 Apr 2019
SRR6576514_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576514_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437432
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7290.16665447429543334No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5480.12527661442235594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8900.051.3348431
TCAACGC12050.037.833014
ATCAACG12100.037.676683
CGATTAA250.001702028437.59899584-85
CAACGCA12300.037.064055
AACGCAG12950.035.5666166
GGATCGT702.1831424E-433.570536
TATCAAC14200.031.7846972
ACGCAGA15550.029.6197857
TACATGG19250.029.0637362
CGCAGAG16350.028.4579568
GTACATG19700.028.45191
ACATGGG19550.027.8867233
GATCTAT856.724319E-427.7064971
GTATAAG2208.54925E-1125.6914791
GCTATAT1351.4550613E-524.3697193
GCAGAGT18800.024.2493539
CATGGGG10950.024.0358874
GAACAGT2602.7284841E-1123.499376
TGGATCG1050.002333563822.3803545
Produced by FastQC (version 0.11.2)