FastQCFastQC Report
Sun 14 Apr 2019
SRR6576515_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576515_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178784
Sequences flagged as poor quality0
Sequence length100
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4330.24219169500626453No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3070.17171558976194737No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2380.13312153212815464No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2150.12025684625022373No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC2050.11466350456416682No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1990.11130749955253266No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT1880.10515482369787006No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC1800.10068015034902453No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATAGA451.6087086E-552.220671
GTATCAA4250.046.445681
TAATAGT450.001011804741.7765354
GCTCTAA450.001011804741.7765351
AACGCAG4750.040.567216
CATGGGT2450.040.2845154
ATGGGTA1650.039.87765
CAACGCA4850.039.7307785
ATCAACG4900.039.3253633
TCAACGC4900.039.3253634
ACATGGG9150.038.5234453
GTACATG9550.037.402031
TACATGG9650.037.0144422
ACGCAGA5600.034.409697
GTCTAGG550.002711298634.18081
TACACCG550.002711298634.18085
CGCAGAG5850.033.7425848
TAAACCA600.004152279331.3324014
TATACCA600.004152279331.3324015
TATCAAC6400.030.8428332