FastQCFastQC Report
Sun 14 Apr 2019
SRR6576515_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576515_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178784
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4870.2723957401109719No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3390.18961428315732953No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3140.17563092894218724No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2730.15269822802935387No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2370.13256219795954896No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2320.12976552711652048No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1980.11074816538392698No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC1950.10907016287810989No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1920.10739216037229282No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAAC250.00515138156.6129761
GGGTACC1850.045.7226527
GTATCAA3750.042.7742461
GTATTGG459.930569E-441.9355351
GGTACCT2200.040.5846258
ACATGGG10050.039.2775043
ATGGGTA1800.039.1606065
GTGTATA500.001667018937.741981
ATGGGGG2000.037.594185
GTACATG10900.037.2225951
TACATGG11050.035.7229772
CATGGGG4850.034.88124
CTGATGT550.00271296134.176534
TCAACGC4700.033.994744
AACGCAG4850.032.9433566
ATCAACG4800.032.30753
CAACGCA5000.031.9550555
CATGGGA3700.031.7518424
GCGGTAT600.0040757531.4516491
GTACCTG2700.031.3284879