FastQCFastQC Report
Sun 14 Apr 2019
SRR6576516_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576516_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149288
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4290.28736402122072774No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2870.19224586035046354No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2400.16076308879481271No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC1980.1326295482557205No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1790.1199024703927978No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT1680.1125341621563689No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA1670.11186431595305718No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC1540.10315631531000481No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGAT200.002161910570.47838694
TTATGAT250.005233488556.3827134
ATGGGTA1700.041.4578745
ATCCTAT500.001697047337.6010741
TAATCAT500.001699844337.5884785
CATGGGT2200.036.3070534
CTCTTAA651.4073681E-436.1548771
GTATCAA3950.035.6972241
ACATGGG8550.031.8732683
GTACATG8450.031.7050461
GGTACCT1658.54925E-1131.323738
TGGGTAC1658.54925E-1131.323736
GGGTACC1658.54925E-1131.323737
TATACTC753.2678785E-431.3237293
CATGGGG4100.030.9417324
TACATGG8750.030.6180132
CTTAATC804.7697593E-429.3659953
ATCAACG4800.028.3871293
TCAACGC4900.027.8077984
CAACGCA5050.026.9818275