FastQCFastQC Report
Sun 14 Apr 2019
SRR6576516_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576516_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149288
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4800.32152617758962543No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3440.23042709393923155No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2860.1915760141471518No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2760.18487755211403462No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2240.1500455495418252No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2070.13865816408552598No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2000.13396924066234392No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT1810.12124216279942127No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT1800.12057231659610952No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC1790.1199024703927978No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG1640.10985477734312202No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1630.1091849311398103No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT1580.10583570012325169No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTATA405.6641485E-446.9943052
CATGGGT2050.045.8481034
GTATCAA3350.043.5894851
ATGGGTA1850.043.1839565
GTAGTAA450.00100009441.8709031
TGGGTAC1850.040.6437236
GGGTACC1850.040.6437237
GGTACCT1900.039.5874148
GTCCTAC500.001678812737.6838151
TATATAC500.001698297737.5954443
ACATGGG8150.034.5970343
GTACATG8500.033.8045961
TACATGG8700.033.4901922
GTACCTG2300.032.7026449
GGTATCA1605.820766E-1132.3845251
GTATATG600.00410449231.4031811
TATGCAG600.00415195831.3295385
TCAACGC4500.031.3295384
AACGCAG4350.031.3295366
ATCAACG4550.030.9852543
Produced by FastQC (version 0.11.2)