FastQCFastQC Report
Sun 14 Apr 2019
SRR6576517_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576517_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences290600
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7700.2649690295939436No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5390.18547832071576048No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4040.13902271163110805No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3910.13454920853406743No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3700.12732278045423262No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3540.12181693048864418No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3390.11665519614590503No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT3050.1049552649690296No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3010.10357880247763249No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2970.10220233998623537No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2910.10013764624913972No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG250.005228894356.408772
GTATCAA6950.056.138231
ATGGGTA2950.041.4230465
ATCAACG9550.040.355363
TCAACGC9700.039.7313044
CAACGCA9650.039.4501275
GTACATG15550.038.996421
AACGCAG9950.038.2606776
GGTACCT2750.037.599388
TACATGG16350.037.0883372
ACATGGG16250.037.0209273
GGGTACC2950.036.6434637
TGGGTAC3100.036.3864976
CATGGGT4800.036.228574
GTGTTAT550.00271161434.1871381
GTATGGG550.00271161434.1871381
ATAGGGG550.002713903334.1812553
ACGCAGA11000.034.1812557
TATCAAC11900.033.5766532
CTAATAG702.181214E-433.5708733