FastQCFastQC Report
Sun 14 Apr 2019
SRR6576517_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576517_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences290600
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7430.2556779077770131No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4910.1689607708189952No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4900.1686166551961459No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4700.16173434273916035No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4120.14177563661390227No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3610.12422573984858913No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3400.1169993117687543No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3290.11321403991741226No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3230.11114934618031659No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3040.10461114934618031No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA5350.063.4133341
TTAGTCG308.8208864E-539.16501638-39
GTACATG14800.038.8418581
ATCAACG8650.038.033083
GTCCTAA500.001678834437.6957051
TAGTCGG250.001701686637.5984240-41
CATGGGT4800.037.2067644
TCAACGC8850.037.1735764
ACATGGG15200.037.10373
CAACGCA9050.036.3520625
AACGCAG9150.035.954776
TACATGG15650.035.7365152
TATCAAC10100.033.038212
ACGCAGA11050.029.7725017
GTGTTAT953.6783407E-529.7597681
ATGGGTA3050.029.2774545
ATAGTAC1154.183594E-628.6074923
CGCAGAG11550.028.4836488
CATGGGG8150.028.256484
TAGTACC1005.31157E-528.1988144